Molecular Biology

                                                   
www.bdbiosciences.com

Gene Expression:

GFP Transfection

 

BD Accuri™ C6 
The BD Accuri™ C6 is a personal flow cytometer that brings flow cytometry within reach by being easy to use, simple to maintain, and affordable.

Flow cytometry can accurately quantitate reporter gene expression (for example, Green Fluorescent Protein, GFP) in each cell in a population being transfected. Co-transfection of a reporter plasmid and a reference plasmid can be very variable in normal human cells, making interpretation difficult in reporter gene assays. However, using flow cytometry, reporter and reference plasmid expression can both be quantitated at the single-cell level, even in cases of low transfection efficiency. In addition, heterogeneity in reporter expression and transient effects in gene expression can be examined.

Since a flow cytometer can simultaneously collect multiparametric data, propidium iodide can be used to monitor the DNA content of cells of varying viabilities, identifying apoptotic cells with sub G1 DNA content. A direct approach of using fluorochrome-conjugated antibodies to the transfected gene product can also be used to screen cell populations. As opposed to bulk detection approaches examining the whole well in a microplate, flow cytometry analysis of individual viable cells can pick up very rare events and can find rare subpopulations.


www.uvp.com

 

UVP manufactures a wide range of pcr cabinets and workstations which provide a clean and contaminant - free environment for the most demanding applications.UVP is also well known for gel documentation systems  as well as other UV related products (see imaging section).


 

 

Through acquisition and ongoing R&D investment, Corning now offers a more comprehensive range of premium, innovative laboratory products and solutions for a wide spectrum of life science applications. Corning’s trusted, quality brands, backed by technical expertise, provide researchers with better ways to advance their research from the beginning of their process to the end.

The Corning®, Costar®, PYREX®,  Axygen®, Gosselin™ and FALCON® brands make Corning a global leader in consumable plastic laboratory tools for life science research.

About Axygen Products

http://www.corning.com/worldwide/en/products/life-sciences/resources/brands/axygen-brand-products.html

Axygen products and solutions for genomics applications are offered through a wide portfolio of tools including: automation tips, PCR consumables, pipet tips, storage plates, sealing options, AxyPrep nucleic acid isolation kits and devices, and benchtop equipment.