Immunology / Immunochemistry

                                       
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Immunology

BD Biosciences supply a variety of conjugated antibodies, purified antibodies, and buffers for cell biology and immunology. These include:

Custom

Immunoassays

BD Biosciences supply a variety of Immunoassay products. They include bead-based immunoassays, ELISA kits, and ELISPOT kits for soluble protein measurements:

Multiplexed quantitation and detection

BD™ Cytometric Bead Array (CBA) is a flow cytometry application that allows users to quantify multiple proteins simultaneously. The BD CBA system uses the broad dynamic range of fluorescence detection offered by flow cytometry and antibody-coated beads to efficiently capture analytes. Each bead in the array has a unique fluorescence intensity so that beads can be mixed and run simultaneously in a single tube. This method significantly reduces sample requirements and time to results in comparison with traditional ELISA and Western blot techniques.

BD Biosciences offers three convenient BD™ ELISA reagent formats for quantitation of cytokines and other soluble proteins.

Individual reagents are comprised of unlabeled capture antibodies, biotinylated detection antibodies, and protein standards offering a wide range of specificities and assay design flexibility. BD OptEIA™ sets include sufficient reagents for five or twenty 96-well plates, and BD OptEIA™ kits contain reagents, as well as pre-coated 96-well plates. All three BD Biosciences sandwich ELISA formats provide fast, easy quantitation of soluble analytes and total response analysis.

BD Biosciences supply Enzyme-linked immunospot assays for detecting and analyzing individual cells that secrete a particular protein in vitro.

Other

BD Biosciences offers in vivo capture assay sets for measuring cellular production of mouse cytokines: IFN-γ, IL-2, IL-4, IL-6, IL-10, and TNF. Specific cytokine-binding monoclonal antibodies form complexes with target cytokines that accumulate in serum and are assayed by enzyme-linked immunosorbent assay (ELISA).